| Product |
XhoI
- MBI |
| Description |
XhoI recognizes the sequence
5'...C/TCGAG...3'
3'...GAGCT/C...5' |
| Storage |
-20°C |
| Concentration |
10u/µl |
| Reaction
Conditions |
Buffer R+
10mM Tris-HCl (pH 8.5), 10mM MgCl2, 100mM KCl and 0.1mg/ml
BSA Incubate at 37°C |
| Storage
buffer |
10mM Tris-HCl (pH 7.4
at 25°C), 100mM KCl, 1mM DTT, 1mM EDTA, 0.2mg/ml BSA
and 50% glycerol |
| Ligation
and Recleavage |
After 50-fold overdigestion
with XhoI, more than 90% of the DNA fragments can be ligated
and recut |
| Methylation
Effect |
XhoI does not cut CTCGm6AG and
m5CTCGAG
CTm5CGAG is cleaved by XhoI at a significantly slower
rate
CG methylation may influence DNA cleavage |
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