| Product |
XbaI
- MBI |
| Description |
XbaI recognizes the sequence
5'...T/CTAGA...3'
3'...AGATC/T...5' |
| Storage |
-20°C |
| Concentration |
10u/µl |
| Reaction
Conditions |
Buffer Y+/TANGO™
33mM Tris-acetate (pH 7.9), 10mM Mg-acetate, 66mM
K-acetate and 0.1mg/ml BSA. Incubate at 37°C |
| Storage
buffer |
10mM Tris-HCl (pH 7.5 at
25°C), 100mM KCl, 10mM
2-mercaptoethanol, 0.1mM EDTA, 0.2mg/ml BSA and 50% glycerol |
| Ligation
and Recleavage |
After 50-fold overdigestion
with XbaI, more than 90% of the DNA fragments can be ligated
and recut |
| Methylation
Effect |
XbaI does not cut TCTAGm6A,
Tm5CTAGA nor does it cut Thm5CTAGA
Blocked by overlapping Dam Methylation |
| Star
Activity |
A large excess of the enzyme
results in star activity |
| Note |
Assay on Lambda DNA (dam-) |
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