| Taq
DNA Polymerase (native) |
| Product |
Taq
DNA Polymerase (native, without BSA) -
MBI |
| Description |
Purified from a thermophilic
bacterium Thermus aquaticus YT1. Taq DNA polymerase catalyzes
5'—3' synthesis of DNA. The enzyme has no detectable
3'—5' proofreading exonuclease activity but possesses
low 5'—3' exonuclease activity. |
| Storage
Buffer |
20mM Tris-HCl
(pH 8.0), 1mM DTT, 0.1mM EDTA, 100mM KCl,
0.5% Nonidet P-40, 0.5% Tween 20 and 50% glycerol. |
| Activity
assay |
67mM Tris-HCl
(pH 8.8 at 25°C), 6.7mM MgCl2, 1mM 2-mercaptoethanol,
50mM NaCl, 0.1mg/ml BSA, 0.75mM activated calf thymus
DNA, 0.2mM of each dNTP, 0.4MBq/ml [3H]-dTTP. |
| Quality
Control |
Tested for
the absence of endo-, exodeoxyribonucleases, ribonucleases
and Polymerase Chain Reaction (PCR). |
| Unit
definition |
One unit
of enzyme catalyzes the incorporation of 10 nanomoles
of deoxyribonucleotides into a polynucleotide fraction
(adsorbed on DE-81) in 30 min. at 70°C. |
| Note |
· Native
Taq DNA polymerase may be preferred for amplifications
involving bacterial DNA sequences homologous to those
found in E.coli.
· The half-life of enzyme
is >40 minutes at 95°C.
· The error
rate of Taq DNA polymerase during PCR is 2.2x10-5 errors
per base pair incorporated (determined according to the
modified method described by Lundberg, K.S., et al). |
| Applications |
Amplification
of DNA fragments by PCR
DNA labeling with radionucleotides, digoxigenin or biotin,
and DNA sequencing. |
|
