| Product |
TaqI |
| Description |
TaqI recognizes the sequence
5'…T/CGA…3'
3'…AGC/T…5' |
| Storage |
-20°C |
| Concentration |
10u/µl |
| Reaction
Conditions |
Buffer TaqI+
10mM Tris-HCl (pH 8.0), 5mM MgCl2, 100mM NaCl and 0.1mg/ml
BSA Incubate at 65°C under paraffin oil in a capped
vial |
| Storage
buffer |
10mM Tris-HCl (pH 7.5 at
25°C), 300mM KCl, 1mM DTT, 0.1mM EDTA, 0.5mg/ml BSA
and 50% glycerol |
| Ligation
and Recleavage |
After 50-fold overdigestion
with TaqI, more than 90% of the DNA fragments can be ligated
and recut |
| Methylation
Effect |
TaqI does not cut TCGm6A
and Thm5CGA, but cuts Tm5CGA
Blocked by overlapping Dam methylation |
| Note |
Assayed using Lambda DNA
(dam-)
Incubation at 37°C results in 10% activity |
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