| Product |
SmaI
- MBI |
| Description |
SmaI recognizes the sequence
5'...CCC/GGG...3'
5'...GGG/CCC...3' |
| Storage |
-20°C |
| Concentration |
10u/µl |
| Reaction
Conditions |
Buffer Y+/TANGO™
33mM Tris-acetate (pH 7.9), 10mM Mg-acetate, 66mM
K-acetate and 0.1mg/ml BSA. Incubate at 30°C |
| Storage
buffer |
10mM Tris-HCl (pH 7.4 at
25°C), 100mM KCl, 1mM DTT, 1mM EDTA, 0.2mg/ml BSA
and 50% glycerol |
| Ligation
and Recleavage |
After 50-fold overdigestion
with SmaI, more than 90% of the DNA fragments can be ligated
and recut |
| Methylation
Effect |
SmaI does not cut m4CCCGGG, m5CCCGGG,
Cm4CCGGG, CCm4CGGG, CCm5CGGG, but cuts Cm5CCGGG
Blocked by CG methylation |
| Star
Activity |
A large excess of the enzyme
results in star activity |
| Note |
Incubation at 37°C
results in 50% activity |
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