| Product |
SalI |
| Description |
SalI recognizes the sequence
5'...G/TCGAC...3'
3'...CAGCT/G...5' |
| Storage |
-20°C |
| Concentration |
10u/µl |
| Reaction
Conditions |
Buffer O+
50mM Tris-HCl (pH 7.5), 10mM MgCl2, 100mM NaCl and 0.1mg/ml
BSA Incubate at 37°C |
| Storage
buffer |
10mM Tris-HCl (pH 7.5 at
25°C), 100mM KCl,
10mM 2-mercaptoethanol, 0.1mM EDTA, 0.2mg/ml BSA and 50%
glycerol |
| Ligation
and Recleavage |
After 50-fold overdigestion
with SalI, more than 90% of the DNA fragments can be ligated
and recut |
| Methylation
Effect |
SalI does not cut GTm5CGAC,
GTCGm6AC, Ghm5UCGAC, but cuts GTCGAm5C
Blocked by CG methylation |
| Star
Activity |
A large excess of the enzyme
results in star activity |
| |
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