| Product |
RsaI
- MBI |
| Description |
RsaI recognizes the sequence
5'...GT/AC...3'
3'...CA/TG...5' |
| Storage |
-20°C |
| Concentration |
10u/µl |
| Reaction
Conditions |
Buffer Y+/TANGO™
33mM Tris-acetate (pH 7.9), 10mM Mg-acetate, 66mM
K-acetate and 0.1mg/ml BSA. Incubate at 37°C |
| Storage
buffer |
10mM Tris-HCl (pH 7.4
at 25°C), 100mM KCl, 1mM DTT, 1mM EDTA, 0.2mg/ml BSA
and 50% glycerol |
| Ligation
and Recleavage |
After 50-fold overdigestion
with RsaI, more than 90% of the DNA fragments can be ligated
and recut |
| Methylation
Effect |
RsaI cuts neither GTm6AC nor GTAm4C
The hemi-methylated sequence GTAm5C is cleaved by RsaI
at a significantly slower rate
Cleavage is blocked when both strands are methylated
as shown
5'...GTAm5C...3'
3'...m5CATG...5'
Overlapping CG methylation may influence DNA cleavage |
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