| Product |
Reverse
Transcriptase (M-MuLV)-MBI |
| Description |
Purified from an E.coli strain carrying
a Molony Murine Leukemia Virus (M-MuLV) Reverse Transcriptase
overproducing Plasmid. The enzyme possesses an RNA and
DNA-directed polymerase activity, and ribonuclease H activity
specific to RNA in RNA-DNA hybrids. |
| Storage |
-20°C |
| Concentration |
200u/µl |
| Content |
Reverse Transcriptase,
5X reaction buffer |
| Unit
definition |
1 unit of enzyme incorporates
1 nanomole of dTMP into a polynucleotide fraction (adsorbed
on DE-81) in 10 min at 37°C. |
| Note |
The synthesized cDNA can
be used for PCR reaction.
For each reaction, 200 units of enzyme must be used. |
