| Product |
PvuII |
| Description |
PvuII recognizes the sequence
5'...CAG/CTG...3'
3'...GTC/GAC...5' |
| Storage |
-20°C |
| Concentration |
10u/µl |
| Reaction
Conditions |
Buffer G+
10mM Tris-HCl (pH 7.5), 10mM MgCl2, 50mM NaCl and 0.1mg/ml
BSA Incubate at 37°C |
| Storage
buffer |
10mM Tris-HCl (pH 7.4 at
25°C), 100mM KCl, 1mM DTT, 1mM EDTA, 0.2mg/ml BSA
and 50% glycerol |
| Ligation
and Recleavage |
After 50-fold overdigestion
with PvuII, more than 90% of the DNA fragments can be
ligated and recut |
| Methylation
Effect |
PvuII cuts neither CAGm4CTG
nor CAGm5CTG |
| Star
Activity |
A large excess of the enzyme
results in star activity |
| |
 |
|
