| Product |
PvuI
- MBI |
| Description |
PvuI recognizes the sequence
5'...CGAT/CG...3'
3'...GC/TAGC...5' |
| Storage |
-20°C |
| Concentration |
5u/µl |
| Reaction
Conditions |
Buffer R+
10mM Tris-HCl (pH 8.5), 10mM MgCl2, 100mM KCl and 0.1mg/ml
BSA Incubate at 37°C |
| Storage
buffer |
10mM Tris-HCl (pH 7.5
at 25°C), 300mM KCl, 1mM DTT, 0.1mM EDTA, 0.2mg/ml
BSA and 50% glycerol |
| Ligation
and Recleavage |
After 50-fold overdigestion
with PvuI, more than 90% of the DNA fragments can be ligated
and recut |
| Methylation
Effect |
PvuI does not cut CGATm4CG,
CGATm5CG, but cuts
CGm6ATCG
Blocked by CG methylation
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