| Product |
PstI |
| Description |
PstI recognizes the sequence
5'...CTGCA/G...3'
3'...G/ACGTC...5' |
| Storage |
-20°C |
| Concentration |
10u/µl |
| Reaction
Conditions |
Buffer O+
50mM Tris-HCl (pH 7.5), 10mM MgCl2, 100mM NaCl and 0.1mg/ml
BSA Incubate at 37°C |
| Storage
buffer |
10mM Tris-HCl (pH 7.4
at 25°C), 200mM NaCl, 1mM DTT, 0.1mM EDTA, 0.15% Triton
X-100, 0.2mg/ml BSA and 50% glycerol |
| Ligation
and Recleavage |
After 50-fold overdigestion
with PstI, more than 90% of the DNA fragments can be ligated
and recut |
| Methylation
Effect |
PstI cuts neither m5CTGCAG,
Chm5UGCAG, CTGm5CAG nor CTGCm6AG
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