| Product |
Proteinase
K -
MBI |
| Description |
Produced by
the fungus Tritirachium album Llimber. It is an endolytic
protease that cleaves peptide bond at the carboxylic sides
of aliphatic, aromatic or hydrophobic amino acids. Proteinase
K is classified as a serine pro-tease. |
| Concentration |
Approximately
600u/ml (approx. 20mg/ml) |
| Unit
Definition |
One unit of
enzyme liberates Folin-positive amino acids and peptides
corresponding to 1µmol tyrosine 1min at 37°C
using denatured hemoglobin |
| Storage
Buffer |
50mM Tris-
HCl (pH 8.0), 5mM CaCl2 and 50% glycerol |
| Quality
Control |
Tested for
the absence of endoexodeoxyribonucleases and ribonucleases |
| Applications |
Inactivation
of DNases and RNases when isolating DNA and RNA from tissues
or cell lines
Determination of enzyme localization
Improving cloning efficiency of PCR products |
