| Product |
NotI |
| Description |
NotI recognizes the sequence
5'...GC/GGCCGC...3'
3'...CGCCGG/CG...5' |
| Storage |
-20°C |
| Concentration |
10u/µl |
| Reaction
Conditions |
Buffer O+
50mM Tris-HCl (pH 7.5), 10mM MgCl2, 100mM NaCl and 0.1mg/ml
BSA Incubate at 37°C |
| Storage
buffer |
20mM Tris-HCl (pH 7.8 at
25°C), 100mM NaCl, 0.1mM EDTA, 10mM 2-mercaptoethanol,
0.02% Triton X-100, 0.2mg/ml BSA and 50% glycerol |
| Ligation
and Recleavage |
After 50-fold overdigestion
with NotI, more than 90% of the DNA fragments can be ligated
and recut |
| Methylation
Effect |
NotI cuts neither GCGGm5CCGC
nor GCGGCm5CGC, but cuts GCGGCCGm5C
Blocked by CG methylation |
| Note |
Assayed on Adenovirus-2
DNA |
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