| Product |
NdeI
- MBI |
| Description |
NdeI recognizes the sequence
5'...CA/TATG...3'
3'...GTAT/AC...5' |
| Storage |
-20°C |
| Concentration |
10u/µl |
| Reaction
Conditions |
Buffer R+
10mM Tris-HCl (pH 8.5), 10mM MgCl2, 100mM KCl and 0.1mg/ml
BSA Incubate at 37°C |
| Storage
buffer |
10mM Tris-HCl
(pH 7.4 at 25°C), 100mM KCl, 1mM DTT, 1mM EDTA, 0.2mg/ml
BSA and 50% glycerol |
| Ligation
and Recleavage |
After 50-fold
overdigestion with NdeI, more than 80% of the DNA fragments
can be ligated and recut |
| Methylation
Effect |
NdeI does
not cut CATm6ATG, but cuts m5CATATG |
| |
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