| Product |
NcoI
- MBI |
| Description |
NcoI recognizes the sequence
5'...C/CATGG...3'.
3'...GGTAC/C...5' |
| Storage |
-20°C |
| Concentration |
10u/µl |
| Reaction
Conditions |
Buffer Y+/TANGO™
33mM Tris-acetate (pH 7.9), 10mM Mg-acetate, 66mM K-acetate
and 0.1
mg/ml BSA. Incubate at 37°C |
| Storage
buffer |
10mM Tris-HCl (pH 7.5 at
25°C), 50mM KCl, 1mM DTT, 0.1mM EDTA, 0.2mg/ml BSA
and 50% glycerol |
| Ligation
and Recleavage |
After 50-fold overdigestion
with NcoI, more than 90% of the DNA fragments can be ligated
and recut |
| Methylation
Effect |
NcoI does not cut m4CCATGG,
m5CCATGG, but cuts CCm6ATGG |
| Star
Activity |
A large excess of the enzyme
results in star activity |
| |
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|
