A qualitative diagnostic kit for detection of Mycobacterium tuberculosis (MTB) DNA in clinical samples such as sputum, urine, and pleural aspiration. Kit contains TB lysis solution for DNA extraction and reagents for Polymerase Chain Reaction.
Part of sputum samples that are decontaminated for culture may be used for PCR. Total processing time for 10 clinical sample is 5 hours. Ready to use PCR mix, positive control and other qualified reagents along with an easy to follow protocol are included. Specific primers amplify conserved region in MTB genome. Using this kit as low as 20 Bacteria/ml of genome could be detected.