| Product |
MspI
(HpaII) - MBI |
| Description |
MspI recognizes the sequence
5'...C/CGG...3'
3'...GGC/C...5' |
| Storage |
-20°C |
| Concentration |
10u/µl |
| Reaction
Conditions |
Buffer Y+/TANGO™
33mM Tris-acetate (pH 7.9), 10mM Mg-acetate, 66mM
K-acetate and 0.1mg/ml BSA. Incubate at 37°C |
| Storage
buffer |
10mM potassium phosphate
(pH 7.5 at 25°C), 200mM NaCl, 1mM DTT, 1mM EDTA, 0.2mg/ml
BSA and 50% glycerol |
| Ligation
and Recleavage |
After 50-fold overdigestion
with MspI (HpaII), more than 90% of the DNA fragments
can be ligated and recut |
| Methylation
Effect |
MspI (HpaII) does not
cut m4CCGG, m5CCGG, Cm4CGG and hm5 Chm5CGG, but cuts
Cm5CGG
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