| Product |
MboI
- MBI |
| Description |
MboI recognizes the sequence
5'... /GATC...3'
3'... CTAG/...5' |
| Storage |
-20°C |
| Concentration |
10u/µl |
| Reaction
Conditions |
Buffer R+
10mM Tris-HCl (pH 8.5), 10mM MgCl2, 100mM KCl and 0.1mg/ml
BSA Incubate at 37°C |
| Storage
buffer |
10mM Tris-HCl (pH 7.5 at
25°C), 50mM KCl, 1mM DTT, 0.1mM EDTA, 0.2mg/ml BSA
and 50% glycerol |
| Ligation
and Recleavage |
After 50-fold overdigestion
with MboI, more than 90% of the DNA fragments can be ligated
and recut |
| Methylation
Effect |
MboI cuts neither Gm6ATC,
GAThm5C nor GAhm5UC, but cuts GATm4C and GATm5C
Blocked by Dam methylation |
| Note |
Assayed using Lambda DNA
(dam-)
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