Lambda DNA is used as
a substrate in restriction enzyme research and in the
testing of restriction endonuclease activity. Phage is
isolated from a heat-inducible lysogenic E.coli W3110
(c/857 sam7 strain) DNA is isolated from purified phage
by phenol extraction and dialyzed against 10mM Tris-HCl
(pH 7.6) and 1mM EDTA. It has a molecular weight of 31.5×106
daltons and contains 48502 base pairs
Storage
Buffer
10mM Tris-HCl (pH 7.6)
and 1mM EDTA
Quality
Control
Gel analysis for purity
and homogeneity of Alw21l, Cpol and
HindIII fragmentation patterns
