| Product |
KpnI
- MBI |
| Description |
KpnI recognizes the sequence
5'...GGTAC/C...3'
3'...C/CATGG...5' |
| Storage |
-20°C |
| Concentration |
10u/µl |
| Reaction
Conditions |
Buffer KpnI+
10mM Tris-HCl (pH 7.5), 10mM MgCl2, 0.02% Triton X-100
and 0.1mg/ml BSA. Incubate at 37°C |
| Storage
buffer |
10mM Tris-HCl (pH 7.5 at
25°C), 50mM KCl, 1mM DTT,
0.1mM EDTA, 0.2mg/ml BSA and 50% glycerol |
| Ligation
and Recleavage |
After 50-fold overdigestion
with KpnI, more than 90% of the DNA fragments can be ligated
and recut |
| Methylation
Effect |
KpnI cuts neither GGTm6ACC,
GGTAm4CC, GGTAm5Cm5C nor GGTACm4C, but cuts GGTAm5CC and
GGTACm5C |
| Star
Activity |
A large excess of the enzyme
results in star activity |
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