Purified from an E.coli
strain carrying a plasmid with the cloned gene encoding
this enzyme.
Klenow Fragment exhibits the 5'—3' polymerase and
the 3'—5' exonuclease activity of DNA polymerase
I, but lacks its 5'-3' exonuclease activity.
Storage
-20°C
Applications
Filling-in
or labeling recessed 3'-termini of double-stranded DNA,
Second strand synthesis of cDNA.
DNA sequencing by the method of Sanger F. et al.
Site-specific mutagenesis of DNA with synthetic
oligonucleotides.
Labeling of DNA by the random primer method
