| Product |
HinfI |
| Description |
HinfI recognizes the sequence
5'...G/ANTC...3'
3'...CTNA/G...5' |
| Storage |
-20°C |
| Concentration |
10u/µl |
| Reaction
Conditions |
Buffer R+
10mM Tris-HCl (pH 8.5), 10mM MgCl2, 100mM KCl and 0.1mg/ml
BSA Incubate at 37°C |
| Storage
buffer |
10mM Tris-HCl (pH 7.5
at 25°C), 100mM KCl, 1mM DTT, 0.1 mM EDTA, 0.2mg/ml
BSA and 50% glycerol |
| Ligation
and Recleavage |
After 50-fold overdigestion
with HinfI, more than 90% of the DNA fragments can be
ligated and recut |
| Methylation
Effect |
HinfI cuts neither Gm6ANTC nor GANThm5C
GANTm5C is cleaved by HinfI at a significantly slower
rate
Overlapping CG methylation may influence DNA cleavage
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