| Product |
HindIII |
| Description |
HindIII recognizes the sequence
5'...A/AGCTT...3'
3'...TTCGA/A...5' |
| Storage |
-20°C |
| Concentration |
10u/µl |
| Reaction
Conditions |
Buffer R+
10mM Tris-HCl (pH 8.5), 10mM MgCl2, 100mM KCl and 0.1mg/ml
BSA. Incubate at 37°C |
| Storage
buffer |
10mM Tris-HCl (pH 7.5
at 25°C), 250mM KCl, 1mM DTT, 0.1mM EDTA, 0.2mg/ml
BSA and 50% glycerol |
| Ligation
and Recleavage |
After 50-fold overdigestion
with HindIII, more than 90% of the DNA fragments can be
ligated and recut |
| Methylation
Effect |
HindIII does not cut m6AAGCTT, AAGm5CTT,
AAGhm5CTT, but cuts Am6AGCTT and AAGChm5Uhm5U |
| |
 |
|
