| Product |
HhaI
(Hin6I)
- MBI |
| Description |
HhaI recognizes the sequence
5'...G/CGC...3'
3'...CGC/G...5' |
| Storage |
-20°C |
| Concentration |
10u/µl |
| Reaction
Conditions |
Buffer Y+/TANGO™
33mM Tris-acetate (pH 7.9), 10mM Mg-acetate, 66mM K-acetate
and 0.1mg/ml BSA. Incubate at 37°C |
| Storage
buffer |
10mM potassium phosphate
(pH 7.4 at 25°C), 100mM NaCl, 1mM EDTA, 7mM 2-mercaptoethanol,
0.2mg/ml BSA, and 50% glycerol |
| Ligation
and Recleavage |
After 50-fold overdigestion
with HhaI (Hin6I), more than 90% of the DNA fragments
can be ligated and recut with this enzyme |
| Methylation
Effect |
HhaI (Hin6I) does not cut
Gm5CGC
Blocked by CG methylation |
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