| GeneRuler™
1Kb DNA Ladder |
| Product |
GeneRuler™
1Kb DNA Ladder -MBI |
| Description |
Plasmids were completely digested with appropriate
restriction endonucleases, phenol extracted, ethanol precipitated
and dissolved in 10mM Tris-HCl (pH 7.6), and 1mM EDTA.
The digests were then mixed. The DNA ladder yields the
following 14 discrete fragments (in base pairs): 10000,
8000, 6000, 5000, 4000, 3500, 3000, 2500, 2000, 1500,
1000, 750, 500, 250.
GeneRuler™ 1 kb DNA ladder was diluted in a loading
dye to a concentration of 0.1mg/ml. The DNA ladder can
be loaded directly on agarose gel. |
| Storage |
-20°C |
| Storage
Buffer |
10mM Tris-HCl (pH 7.6),
10mM EDTA. |
| Note |
· The bright band
at 3000bp serves as a reference.
· Apply 1µl
of DNA ladder per 1mm gel lane width.
· Do
not heat before loading. |
|
