| Product |
EcoRV
(Eco 321) - MBI |
| Description |
EcoRV recognizes the sequence
5'...GAT/ATC...3'.
3'...CTA/TAG...5'. |
| Storage |
-20°C |
| Concentration |
10u/µl |
| Reaction
Conditions |
Buffer R+
10mM Tris-HCl (pH 8.5), 10mM MgCl2, 100mM KCl and 0.1mg/ml
BSA Incubate at 37°C |
| Storage
buffer |
25mM Tris-HCl (pH 7.5
at 25°C), 200mM NaCl, 1mM DTT, 1mM EDTA, 0.2mg/ml
BSA and 50% glycerol |
| Ligation
and Recleavage |
After 50-fold overdigestion
with EcoRV (Eco 321), more than 90% of the DNA fragments
can be ligated and recut |
| Methylation
Effect |
EcoRV (Eco 321) cuts
GATATm5C |
| Star
Activity |
A large excess of the enzyme
results in star activity |
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