| Product |
EcoRI |
| Description |
EcoRI recognizes the sequence
5'...G/AATTC...3'
3'...CTTAA/G...5' |
| Storage |
-20°C |
| Concentration |
10u/µl |
| Reaction
Conditions |
Buffer O+
50mM Tris-HCl (pH 7.5), 10mM MgCl2, 100mM NaCl and 0.1mg/ml
BSA Incubate at 37°C |
| Storage
buffer |
10mM potassium phosphate
(pH 7.4 at 25°C), 300mM NaCl, 1mM EDTA, 7mM 2-mercaptoethanol,
0.2mg/ml BSA, 0.15% Triton X-100 and 50% glycerol |
| Ligation
and Recleavage |
After 50-fold overdigestion
with EcoRI, more than 90% of the DNA fragments can be
ligated and recut |
| Methylation
Effect |
EcoRI cuts neither Gm6AATTC
nor GAm6ATTC, but cuts GAATTm5C, GAATThm5C andGAAhm5Uhm5UC |
| Star
Activity |
Low salt concentration,
large excess of the enzyme, high pH, or the replacement
of Mg2+ by Mn2+ results in star activity |
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