| Product |
DraI
(AhaIII) - MBI |
| Description |
DraI recognizes the sequence
5'...TTT/AAA...3'
3'...AAA/TTT...5' |
| Storage |
-20°C |
| Concentration |
10u/µl |
| Reaction
Conditions |
Buffer B+
10mM Tris-HCl (pH 7.5), 10mM MgCl2, and 0.1mg/ml BSA
Incubate at 37°C |
| Storage
buffer |
10mM Tris-HCl (pH 7.5
at 25°C), 50mM KCl, 1mM DTT, 0.1mM EDTA, 0.2mg/ml
BSA and 50% glycerol |
| Ligation
and Recleavage |
After 50-fold overdigestion
with DraI, approximately 90% of the DNA fragment can be
recut |
| Methylation
Effect |
DraI dos not cuts TTTAm6A blocked by overlapping EcoKI methylation |
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