| Product |
BglII
- MBI |
| Description |
BglII recognizes the sequence
5'...A/GATCT...3'
3'...TCTAG/A...5' |
| Storage |
-20°C |
| Concentration |
5u/µl |
| Reaction
Conditions |
Buffer O+
50mM Tris-HCl (pH 7.5), 10mM MgCl2, 100mM NaCl and 0.1mg/ml
BSA Incubate at 37°C |
| Storage
buffer |
10mM Tris-HCl (pH 8.2
at 25°C), 200mM KCl, 1mM DTT, 0.1mM EDTA, 0.5mg/ml
BSA and 50% glycerol |
| Ligation
and Recleavage |
After 50-fold overdigestion
with BglII, more than 90% of the DNA fragments can be ligated
and recut |
| Methylation
Effect |
BglII does not cut AGATm5CT and
AGAThm5CT,
but cuts AGm6ATCT and AGAhm5UChm5U |
| Inactivation |
10mM EDTA |
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