| Product |
BglI - MBI |
| Description |
BglI recognizes the sequence
5'...GCCNNNN/NGGC...3'
3'...CGGN/NNNNCCG...5' |
| Storage |
-20°C |
| Concentration |
10u/µl |
| Reaction
Conditions |
Buffer O+
50mM Tris-HCl (pH 7.5), 10mM MgCl2, 100mM NaCl and 0.1mg/ml
BSA Incubate at 37°C |
| Storage
buffer |
10mM Tris-HCl (pH 7.5
at 25°C), 300mM NaCl, 1mM DTT, 0.1mM EDTA, 0.2mg/ml
BSA and 50% glycerol |
| Ligation
and Recleavage |
After 50-fold overdigestion
with BglI, more than 90% of the DNA fragments can be ligated
and recut |
| Methylation
Effect |
BglI does not cut Gm5CCN5GGC and
GCm4CN5GCC,
but cuts GCm5CN5CN5GGC
The hemi-methylated sequence GCCN5GGm5C is cleaved by
BglI at a significantly slower rate
Cleavage is blocked when both strands are methylated as
shown
5'...GCCN5GGm5C...3'
3'... m5CGGN5CC G...5'
Overlapping GC methylation may influence DNA cleavage |
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