| Product |
BclI - MBI |
| Description |
BclI recognizes the sequence
5'...T/GATCA...3'
3'...ACTAG/T...5' |
| Storage |
-20°C |
| Concentration |
10u/µl |
| Reaction
Conditions |
Buffer G+
10mM Tris-HCl (pH 7.5), 10mM MgCl2, 50mM NaCl and 0.1mg/ml
BSA Incubate at 55°C |
| Storage
buffer |
10mM Tris-HCl (pH 7.4
at 25°C), 100mM KCl, 1mM DTT, 1mM EDTA, 0.2mg/ml BSA
and 50% glycerol |
| Ligation
and Recleavage |
After 50-fold overdigestion
with BclI, more than 90% of the DNA fragments can be ligated
and recut |
| Methylation
Effect |
BclI cuts neither TGm6ATCA
nor TGAThm5CA, but cuts TGATm5CA.
Blocked by Dam methylation |
| Star
Activity |
Star activity is observed
at a greater than 10-fold overdigestion substrate with
BclI. |
| Note |
Incubation at 37°C
results in 50% activity
Assayed using Lambda DNA (dam-) |
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