| Product |
BamHI |
| Description |
BamHI recognizes the sequence
5'...G/GATCC...3'
3'...CCTAG/G...5' |
| Storage |
-20°C |
| Concentration |
10u/µl |
| Reaction
Conditions |
Buffer BamHI+
10mM Tris-HCl (pH 8.0), 5mM MgCl2, 100mM KCl, 0.02% Triton
X-100 and 0.1mg/ml BSA. Incubate at 37°C |
| Storage
buffer |
10mM Tris-HCl (pH 7.4
at 25°C), 100mM KCl, 1mM DTT, 1mM EDTA, 0.2mg/ml BSA
and 50% glycerol |
| Ligation
and Recleavage |
After 50-fold overdigestion
with BamHI, more than 90% of the DNA fragments can be
ligated and recut |
| Methylation
Effect |
BamHI does not cut GGATm4CC,
GGATm5CC,
GGAhm5UCC, but cuts GGATCm5C, GGm6ATCC,
GGm6ATCm5C and GGATCm4C |
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