| Product |
AluI
- MBI |
| Description |
AluI recognizes the sequence
5'...AG/CT...3'
3'...TC/GA…5' |
| Storage |
-20°C |
| Concentration |
10u/µl |
| Reaction
Conditions |
Buffer Y+/TANGO™
33mM Tris-acetate (pH 7.9), 10mM Mg-acetate, 66mM K-acetate
and
0.1mg/ml BSA. Incubate at 37°C |
| Storage
buffer |
10mM Tris-HCl (pH 7.5
at 25°C), 50mM KCl, 1mM DTT, 0.1mM EDTA, 0.2mg/ml
BSA and 50% glycerol |
| Ligation
and Recleavage |
After 50-fold overdigestion
with AluI, approximately more than 90% of the DNA fragments
can be ligated and recut |
| Methylation
Effect |
AluI cuts neither m6AGCT,
AGm4CT, AGm5CT nor AGhm5CT |
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